Evaluation of Oxidative Stress and Using a Novel Automated Method
For Measurement of Total Antioxidant Status in Preeclampsia
Ebru KALE1, Ahmet KALE2, Naime CANORUÇ1, Nurten AKDENİZ2, Ahmet YALINKAYA2
Diyarbakır-Turkey
OBJECTIVE: The purpose of this study was to evaluate total antioxidant status of preeclampsia with using a more recently developed automated measurement method and measurement of malondialdehyde (MDA) levels as an oxidative stress marker in women with preeclampsia
STUDY DESIGN: We performed a case-control study consisting of randomly selected 50 healthy pregnant women and 50 patients diagnosed as preeclampsia. We measured the total plasma antioxidant capacity for all patients and oxidative stress was evaluated with measurement of red blood cell malondialdehyde (MDA) levels.
RESULTS: The serum total antioxidant response (TAR) levels were lower (1.29±0.33 TAR mmol Trolox eq/L) and the red blood cell malondialdehyde levels of (MDA) were higher (7.1±0.4 nmol MDA/l) before delivery (P<0.001) and these levels were not different after delivery (six weeks later) in preeclampsia than control group (P>0.05).
The serum total antioxidant response (TAR) levels significantly increased (1.55±0.49 mmol Trolox eq/L) and the malondialdehyde levels significantly decreased (2.9±0,6 nmol MDA/l) in preeclamptic women after delivery when compared with the pre delivery levels
CONCLUSIONS: Our findings suggest that preeclampsia is related with enhanced lipid peroxidation and decreased total antioxidant response before delivery and these parameters may be related at least partly to the pathogenesis of preeclampsia. Decreased lipid peroxidation and increased total antioxidant response after delivery may suggest recovery of preeclampsia and using a simple, rapid and reliable automated method facilitates to evaluate and follow up of the levels of oxidative stress in preeclampsia and this novel method may be used as a routine test in clinical practice while managing preeclampsia
(Gynecol Obstet Reprod Med 2006; 12:000-000)
Key Words: Preeclampsia, Oxidative stress, Total antioxidant response, Malondialdehyde
